Microalgae are sunlight driven cell factories that convert carbondioxide to potential biofuels. The green algae botryococcus braunii is widely recognized as a source of oil, including hydrocarbons. Colony organization in the green alga botryococcus braunii. Cultivation of botryococcus braunii strain in relation of. Botryococcus braunii, a green colonial microalga, is an unusually rich renewable source of hydrocarbons and other chemicals. Botryococcus braunii is a green colonial microalga, which is approximately 10. Harvesting and preextraction processing of the algal biomass are among the major challenges for commercial sustainability of algaebased biofuel production. Identification of unique mechanisms for triterpene. The performance of supercritical co2 extraction of lipids from botryococcus braunii for biodiesel production was studied.
Botryococcus braunii is a green alga with high long chain hydrocarbon content suitable as a source of biofuel. Genome sequence of fuelproducing alga botryococcus. The experiments were conducted at 5080c and 200250 bar. Liquid hydrocarbon production potential of a novel strain. Economic efficiency of different light wavelengths and. Transesterification cannot be used to make biodiesel from botryococcus. Evaluation of media and light source effects on the growth. Botryococcus braunii is a microalga with an extracellular hydrocarbon matrix, which makes it an appropriate green energy source. Botryococcus braunii is member of the chlorophyceae chlorophyta. Transcritome analysis of botryococcus braunii race a and. Hydrocarbons can constitute up to 75% of the dry mass of b. Botryococcus braunii for biodiesel production sciencedirect. Several novel methods are described to advance the study of botryococcus braunii race b as a potential production platform for algae biofuels. Co2 removal from industrial flue gas using botryococcus.
Summarizing the nature of this alga is fundamental to establish the detail environment conditions for optimal biomass growth and the production of lipids, as well as upstream processing technologies for biodiesel. The vast majority of these hydrocarbons are botryocuccus oils. Botryococcus braunii, microalgae, bubble column photo bioreactor, biofuels. Devarenne said that because only portions of the b. Production of biofuel by using micro algae botryococcus braunii. Pdf isolation and cultivation of botryococcus braunii. Botryococcus braunii an overview sciencedirect topics. Biodiesel synthesis by direct transesterification of microalga botryococcus braunii with continuous methanol reflux. A new mechanism for efficient hydrocarbon electroextraction from.
Growth and photosynthetic activity of botryococcus braunii. Lipid accumulation in the extracellular space in b. Enhancement of biomass and hydrocarbon productivities of. Direct transesterification of microalga botryococcus. Sequence analysis of the small subunit of rrna 16s rna and its comparison with the sequences for other algae suggests that b.
Acoi 58 and acoi 1257, two different portuguese strains of colonial green microalga botryococcus bruanii kutz obtained from coimbra collection of algae acoi, were evaluated on their potential for biofuel production, which was assessed from batch. Colonies held together by a lipid biofilm matrix can. The green alga botryococcus braunii has high hydrocarbon content, ranging from 15 to 75% of dry weight, as long chain unsaturated hydrocarbons sawayama et al. Botryococcus braunii is a green, microalgae known for its high hydrocarbon content.
Systematic metabolic characterization of hydrocarbon and exo. Think of it as a 166 millionpiece jigsaw puzzle, he said, given that the size of the b. The green microalga botryococcus braunii produces hydrocarbon oils at 2575% of its dry weight and is a promising source of biofuel. Therefore, it is essential to conduct a comprehensive study about microalgae. Studies on batch and continuous cultures of botryococcus. Biodiesel from algae is a promising solution in the field of green energy. Botryococcus braunii bb is a green, pyramidshaped planktonic microalga that is of potential importance in the field of biotechnology. Botryococcus braunii is characterised by an apparent ability to synthesise and accumulate a wide variety of reserv e substances with the most promising composition for biofuel production. This study focuses on extracting oil from the microalgae matrix rather than the cells, eliminating the need for an excessive electric field to create electropermeabilization. Characterization of oilextracted residue biomass of. Botryococcus braunii for biodiesel production request pdf.
Active hydrocarbon biosynthesis and accumulation in a. Botryococcus braunii is a green microalga capable of producing large amounts of external longchain hydrocarbons suitable as a source of biofuel. This study focuses on extracting oil from the microalgae matrix. Biodiesel from algae oil after the extraction processes, the resulting microalgal oil can be converted into biodiesel through a process called transesterification. Cell morphology and biodiversity of botryococcus braunii. Pdf evaluation of two portuguese strains of botryococcus. The growing shortage of fossil fuels caused an increase in the demand for alternative and renewable fuels. Due to its external hydrocarbon production, mass cultivation of b. The colonial microalgae botryococcus braunii as biorefinery. Phylogenetic placement of botryococcus braunii trebouxiophyceae and botryococcus sudeticus isolate utex 2629 chlorophyceae1 hoda h.
Botryococcus braunii is a colonial green alga whose cells associate via a complex extracellular matrix ecm and produce prodigious amounts of liquid hydrocarbons that can be readily converted into. Botryococcus braunii bb is a green, pyramid shaped planktonic microalga of the order chlorococcales class chlorophyceae that is of potentially great importance in the field of biotechnology. Botryococcus braunii, microalgae, bubble column photo bioreactor, biofuels, saccharomyces cerevisiae, soxhlet apparatus. Green microalga botryococcus braunii has relatively higher lipid content than other microalgae. This characteristic has attracted increasingly more attention in the last two decades in attempts to exploit b. The subisolate belongs to the group of botryococcus braunii race a. Botryococcus braunii as a potential candidate for the.
Faculty of agriculture, trakia university, stara zagora, bulgaria. In this study, we have attempted to analyze the transcriptome data of two races of b braunii. Mechanical cell disruption of microalgae for investigating. An optimized method for the bioharvesting of microalgae. An energyefficient and costeffective method of in situ recovery of the hydrocarbonrich oil from the harvest aqueous culture of b. The colonial green microalga botryococcus braunii is found in freshwater and brackish ponds and lakes around the world, where it often can be found in large floating masses. Pdf biodiesel synthesis by direct transesterification of.
As a potential source of biofuel, the green colonial microalga botryococcus braunii produces large amounts of hydrocarbons that are accumulated in the extracellular matrix. Establishment and maintenance of axenic botryococcus. Microalgae biodiesel are reported to be better than fossil fuels in terms of lifecycle energy performance. The present study focused on the cultivation of botryococcus braunii with the fedbatch in 4 l lab. Biofuel biodiesel, produced from microalgae such as botryococcus braunii is an alternative energy source, that is renewable because algae can be cultured as needed, more biodegradable with lower. Cultivation of botryococcus braunii strain in relation of its use for biodiesel production abstract authors address. Evaluation of two portuguese strains of botryococcus.
Botryococcus braunii as a potential candidate for the waste water treatment and hydrocarbon accumulation g. The green alga botryococcus braunii shows great potential for biodiesel. Thus this investigation was undertaken to grow the cultures of botryococcus braunii in photobioreactor and for the biofuels production. Botryococcus braunii is considered a promising source of nonoxygenated liquid biofuels. High biofuel production of botryococcus braunii using optimized cultivation strategies.
Acoi 58 and acoi 1257, two different portuguese strains of colonial green microalga botryococcus bruanii kutz obtained from coimbra collection of algae acoi, were evaluated on their potential for biofuel. Biodiesel is an alternative fuel source that is obtained from renewable biomass, such as canola, soybean, corn, palm, sunflower, and algae. Pdf business evaluation of a green microalgae botryococcus. Green alga, botryococcus braunii symbolizes one of the most favorable resources of biodiesel due to relatively high lipid content. Finally, this merged network can be used to study acclimation processes in the consortium. Botryococcus braunii is a microalga with an extracellular. There have been several studies indicating that cultures of b. A feedforward culturing strategy to obtain and maintain highdensity cultures of b.
Application of nacl for cultivation of isolated botryococcus braunii strains 229 2. Colonies held together by a lipid biofilm matrix can be found. Botryococcus braunii is a green, pyramidshaped planktonic microalga that is of potentially great importance in the field of biotechnology. Botryococcus braunii, chlamydomonas and desmococcus olivaceous are some of the algal species which are targeted for various reasons. Pdf business feasibility of oil production using the green alga botryococcus braunii has.885 777 260 915 1078 467 1475 499 1450 1291 409 757 1056 280 1150 1068 998 1492 823 1083 177 639 987 1031 162 1010 640 42 1088 92 755 547