A dual pressure linear ion trap orbitrap instrument with very high. Foundation, ion maxs, ltq xl, orbitrap elite, orbitrap velos pro, velos, velos pro, wideband activation, and zoomscan are. Only use fuses of the type and current rating specified. Thermo fisher ltq velos mass spectrometer quadrupole. Calibration is not hard but still takes a while and has to be done every two weeks. Both instruments were run with peptide recognition mode enabled, but exclusion of singly charged and unassigned precursor ions was only enabled on the ltq orbitrap velos. The ltq orbitrap velos getting startedcontains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed. The orbitrap velos is far one of the fastest mass spectrometers and is able to be coupled to capillary electrophoresis. Orbitrap fusion series mass spectrometer we know you, or someone you know, worked very hard to get your orbitrap based mass spectrometer in your lab and even harder preparing your precious samples.
The orbitrap tribrid series hardware manual describes the modes of operation and hardware components for the thermo scientific orbitrap tribrid series mass spectrometer ms system. Thank you for watching gentech scientific visit our site. Thermo scientific orbitrap velos pro hybrid ms from. The orbitrap velos pro is a member of the family of ltq mass spectrometer ms hybrid instruments. Ions generated by api are collected in the ltq xl followed by axial ejection to the cshaped storage trap which is used to store and collisionally. Cid, pqd, etd, and now traphcd fragmentation increases flexibility for structural elucidation fast, sensitive ion trap for the most demanding applications generation ii ion optics improve robustness and reduce downtime. Metabolite analysis untargeted metabolomics and metabolite structural elucidation. This orbitrap elite getting started manual provides information on how to set up, calibrate, and tune the orbitrap elite mass spectrometer. Thermo scientific ltq orbitrap velos hy b rid ft mass.
Schematic of the ltq orbitrap velos ms instrument with. Ltq orbitrap xl offering outstanding mass accuracy, resolving power, dynamic range, and high sensitivity the hybrid ft mass spectrometer combines a linear ion trap ms and the orbitrap mass analyzer. Dartthermo ion max vapur interface manual si140aidc rev 1 for thermo ltq orbitrap velos, ltq orbitrap xl, ltq ion trap, exactive series high performance, and all other instruments that utilize the ion max source version 20121023 ionsense inc. Ltq orbitrap velos with etd thermo fisher scientific applications. Instrument configuration gives instructions about configuring the instrument.
Metabolome identification using lcmsn orbitrap based mass spectrometry. Schematic of the ltq orbitrap velos ms instrument with openi. Thermo fisher ltq velos mass spectrometer quadrupole orbitrap analyzer housing assembly. Thermo orbitrap elite and velos pro mass spectrometer. Velos exit sign consist of 2 parts, the psu unit and the emergency sign. Summary of contents for thermo scientific orbitrap fusion lumos page 1 orbitrap tribrid series getting started guide 8000097026 revision a june 2018. A full calibration and data verification is performed ensuring our used orbitrap systems are returned to excellent working condition. Q exactive hybrid quadrupole orbitrap ms with zipchip system a comprehensive ceesims solution for biopharma applications. Thermo scientific ltq xl getting started pdf download. For the ltq xl and ltq velos ms detectors compatible with ltq orbitrap xl and ltq orbitrap velos.
Also, this symbol appears on the instrument to refer the user to instructions in this manual. Dartthermo ltq orbitrap interface manual for thermo ltq orbitrap velos, ltq orbitrap xl, ltq ion trap, exactive high performance, and all other instruments that utilize the ion max source version 20100814 ionsense inc. Thermo fisher scientific orbitrap velos pro hardware manual pn 1288290, revision a i read this first welcome to the thermo scientific orbitrap velos pro system. The three major novel hardware elements are i the introduction of a more efficient ion transfer system stacked ring ion guide or slens, ii a dual pressure ion trap, and iii a more efficient hcd cell fig. Details about thermo fisher ltq velos mass spectrometer quadrupole orbitrap analyzer housing. Linear trap quadropole ltq orbitrap velos mass spectrometry.
Thermo scientific pierce ltq velos esi positive ion. Thermo scientific ltq orbitrap velos hy b rid ft mass spectrometer performance speci fications performance characteristics. Ltq velos, nanospray flex, nanoviper, orbitrap elite, orbitrap velos pro, proteome. Wheels at the bottom side of the instrument facilitate positioning the ltq orbitrap xl at the intended place in the laboratory. Rp54troubleshooting liquid chromatography tandem mass spectrometry lcmsms systems is a top priority in academic and commercial proteomics laboratories. Procedures in chapters 14 can be performed from the xcalibur tune plus window. So, you actually have ability of what you can do with a qtrap and additionally have an. Ltq orbitrap xl hybrid ion traporbitrap mass spectrometer. The instrument is designed to be placed with it s rear panel against a wall. The data was generated by hplc apparatus agilent 1200 series, ltq orbitrap xl thermofisher scientific. Ltq orbitrap xl getting started, orbitrap velos pro getting started, or orbitrap elite.
Obtain high sensitivity full scan ms along with indepth msn crm capabilities with the thermo scientific ltq xl linear ion trap mass spectrometer. Ltq orbitrap xl hybrid ion trap mass spectrometer support. This ltq orbitrap velos getting started manual provides information on how to set up, calibrate, and tune the ltq orbitrap velos. Exactive series systems are members of the thermo scientific family of mass spectrometer ms detectors that are powered by orbitrap technology. We have 2 thermo scientific ltq xl manuals available for free pdf download. While there are two positive solutions, there is only one negative solution. Dartthermo ltq orbitrap interface manual for thermo ltq orbitrap velos, ltq orbitrap xl, ltq ion trap, exactive high performance, and all other instruments that utilize the ion max source version 20120423 ionsense inc. About this guide this ltq orbitrap xl getting started manual provides you with information on how to set up, calibrate, and tune the ltq orbitrap xl. Page 42 45 degrees counterclockwise to free the probe from the alignment notch.
Who uses this guide this orbitrap elite getting started manual is. Orbitrap velos has an ion trap in the front and an orbitrap analyzer at the rear end of the instrument. Routine maintenance is also addressed and filters, liners, tubing, etc. Etd module hardware manual for velos pro and ltq xl mass spectrometers compatible with orbitrap velos pro, ltq orbitrap velos, orbitrap elite, and ltq orbitrap xl 9800097004 revision d. Both orbitrap and fticr instruments can accumulate longer transients, with fticr being able to. In addition, this manual provides stepbystep instructions for. The orbitrap velos pro mass spectrometer is a member of the family of ltq mass spectrometer ms detectors.
The thermo fisher scientific ltq orbitrap velos is a unique hybrid fourier transform mass spectrometer. These appear throughout the manual, where applicable, and are defined in. Thermo scientific exactive series operating manual pn bre0012255, revision a 11 chapter 1 using this manual welcome to the thermo scientific exactive series system. Thermo scientific ltq xl manuals manuals and user guides for thermo scientific ltq xl. Orbitrap velos pro getting started thermo fisher scientific.
In addition, this manual provides stepbystep instructions for cleaning and maintaining your instrument. Qexactive outperformed ltq orbitrap velos hcd in terms of number of peptide and protein groups, due to faster scan rate and higher resolution. Warning symbols alert the user to check for hazardous conditions. The thermo ltq orbitrap velos mass spectrometer is a hybrid mass spectrometer incorporating the ltq velos dual cell linear trap and the orbitrap analyzer.
Mass spectrometrybased proteomics using q exactive, a. It also provides the instruments cleaning and maintenance procedures. A collision cell for performing higher energy cid experiments. The pierce ltq velos esi positive ion calibration solution is a readytouse liquid formulation ideal for quickly performing the routine calibration required to maintain the robust performance of thermo scientific mass spectrometers. Orbitrap mass spectrometry american chemical society. For the ltq orbitrap velos the lower threshold for targeting a precursor ion in the ms scans was 5,000 counts. Troubleshooting ltq and ltqorbitrap lcmsms systems with massqc. Operating modes of the instrument samples can be introduced into the api source at the front side by a variety of methods including direct infusion via a syringe pump or an uhplc system thermo scientific accela. About this guide this ltq orbitrap xl etd hardware manual contains a description of the modes of operation and principle hardware components of your ltq orbitrap xl etd instrument. Orbitrap fusion lumos orbitrap fusion and orbitrap idx orbitrap fusion lumos low voltage directive 201435eu this device complies with low voltage directive 201435eu and the harmonized safety standard iecencsa ul 610101, 3rd edition. The new hcd cell in the ltq orbitrap velos is more efficient, resulting in improved quantitation of isobarically labeled peptides, such as applications requiring tandem mass tags tmt. About this guide this orbitrap velos pro getting started manual provides information on how to set up, calibrate, and tune the orbitrap velos pro mass spectrometer.
The velos pro was custom designed for both qualitative and quantitative applications in the laboratory. The ltq orbitrap xl is a member of the family of ltq mass spectrometer ms detectors. David peake, ioanna ntai, amanda souza and ralf tautenhahn. We are working just as hard to make sure that you have the latest information and all the additional resources you need to produce the highest. Q exactive hybrid quadrupole orbitrap mass spectrometer. In practical traps, the outer electrode is sustained at virtual ground and a voltage of 3.
Q exactive hybrid quadrupole orbitrap mass spectrometer support. Ltq orbitrap xl hybrid ion trap orbitrap mass spectrometer support troubleshooting find valuable information. Instructions are available to fix an issue with the etd reagent intensity on orbitrap fusion only systems. Ltq orbitrap velos is a hybrid mass spectrometer incorporating the ltq velos dual cell linear trap and the orbitrap analyzer. Ltq orbitrap xl hardware manual thermo fisher scientific. Thermo scientific orbitrap fusion lumos getting started manual. View and download thermo scientific etd getting started manual online. Achieving peak instrument performance reference manual for the ltq velos, velos pro, ltq orbitrap velos, orbitrap velos pro, and orbitrap elite getting started guide. Emc directive 201430eu and other emc test standards.
We present a new software tool, massqc, that builds on work from the national institute of standards. A, the stacked ring ion guide slens increases the ion flux from the. Welcome to the thermo scientific orbitrap velos pro system. Who uses this guide this orbitrap elite getting started manual is intended for all personnel. Note the following procedures assume that you are familiar with your instrument and software. Ion max and ion maxs api source hardware manual or hesiii probe user. Ltq orbitrap currently the orbitrap analyzer exists in two variants. Velos pro dualpressure linear ion trap mass spectrometer. Explore our getting started and troubleshooting sections for solutions to top inquiries and common problems. About this guide this ltq orbitrap velos hardware manual contains a description of the modes of operation and principle hardware components of your ltq orbitrap velos instrument.
Procedures in chapters 14 can be pe rformed from the xcalibur tune plus window. In order to compare ltq orbitrap velos hcd and qexactive for proteomic analysis of 1 ng raw 264. In the ltq orbitrap velos mass spectrometer, a dualpressure ion trap assembly. Pulsed q dissociation pqd for generating more fragments and extending the low mass range. B, the dual linear ion trap design enables efficient trapping and activation in the highpressure cell left and fast scanning and detection in the low pressure cell. For the ltq velos, velos pro, ltq orbitrap velos, orbitrap velos pro, and orbitrap elite. Automatic gain control, data dependent, foundation, ion maxs, ltq xl, orbitrap elite, orbitrap velos pro, velos, velos pro, wideband activation, and zoomscan are trademarks, and ion max. Comparison of ltqorbitrap velos and qexactive for proteomic. These appear throughout the manual, where applicable. All information in this guide concerning the orbitrap velos pro mass. The ltq orbitrap xl hardware manualcontains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed.
Welcome to the thermo scientific ltq orbitrap xl etd system. Jan 15, 20 the ltq orbitrap velos and qexactive mass spectrometers were compared for shotgun proteomic analysis of 1 to 1,000 ng raw 264. Ltq series getting started guide thermo fisher scientific. Superior mass accuracy, mass resolution, dynamic range, detection power, high productivity, and low maintenance. The ltq xl ion trap ms extends the legendary ms n performance of the ltq ion trap, incorporating more techniques to generate structural information through innovations in thermo scientific ion trap technology unparalleled sensitivity and unmatched ms n spectral quality. Mass spectrometry analysis on the ltq orbitrap velos. This is achieved by vacuum interface using a stacked ring radio frequency ionguide with 10fold higher transfer efficiency in msms mode and 35 fold in full scan spectra, by a dual pressure ion trap configuration, and by reduction of overhead times between scans. Comparison of ltq orbitrap velos hcd with qexactive. Ltq orbitrap velos getting started thermo fisher scientific. After processing peaks with xcms online tool i did annotations with different databases. For the qexactive, two methods were used in the manuscript. Protein analysis bottomup proteomics, topdown proteomics, post translational modification ptm analysis and relative quantitation. Q exactive hybrid quadrupoleorbitrap mass spectrometer support. The high mass accuracy of the ltq orbitrap velos increases the speed and con.
Ltq orbitrap xl is a member of the family of ltq mass spectrometer ms hybrid instruments. Reference manual for the ltq velos, velos pro, ltq orbitrap velos. Exactive plus operating manual planet orbitrap mafiadoc. Ltq orbitrap velos increases the speed and confidence of. Note thermo fisher scientific no longer ships the ltq velos and lxq mass spectrometers. Thermo scientific ltq velos dualpressure linear ion trap 2 the thermo scienti. Ltq orbitrap velos getting started concordia university. The ltq velos esi positive ion calibration solution can be used to calibrate the ltq velos velos pro, the ltq orbitrap velos orbitrap velos pro, the orbitrap elite, and the qexactive mass spectrometer instruments. Thermo scientific ltq xl orbitrap lcms system ebay. Orbitrap velos pro hardware manual thermo fisher scientific. A single quadrupole offers selected ion monitoring. Electronic board burnt down twice in our first years use.
Building on a tradition of innovation, the ltq velos provides the analyst with signi. The ltq orbitrap xl etd is a member of the family of ltq mass spectrometer ms hybrid instruments. Psus velos psu 1,5h nicd velos psu 3h nicd velos psu mains with vcgs2 velos psu mains velos psu 1h nimh autotest velos psu 3h nimh autotest order code description oesc oesc. Ltq orbitrap velos is the next generation mass spectrometers, thanks to its significantly increased sensitivity and scan speed. Welcome to the thermo scientific, ltq orbitrap xl system. The ltq orbitrap velos instrument described in this paper is a further development of the ltq orbitrap product 10. Warn ing symbols alert the user to check for hazardous conditions. This was because of the higher sequencing speed of the q. The ltq orbitrap is a high performance lcms and msn system, combining rapid ltq ion trap data acquisition with high mass accuracy orbitrap mass analysis. This orbitrap velos pro hardware manual contains a description of the modes of operation and principle hardware components of your orbitrap velos pro instrument. Proteomics using thermo scientific orbitrap velos pro and. Quick reference guide achieving peak instrument performance for the ltq velos, velos pro, ltq orbitrap velos, orbitrap velos pro, and orbitrap elite. Mass spectrometer thermo scientific ltq orbitrap velos.
The source is similar to the commercial source of the thermo scientific ltq velos. In order to make your choice you need to add 2 lines in your ordering for both parts of velos. Orbitrap velos pro building on the industryleading thermo scientific orbitrap platform, the thermo scientific orbitrap velos pro hybrid ion trap orbitrap mass spectrometer features dualpressure linear ion trap technology that delivers increased sensitivity and dynamic range with ultimate robustness. Thermo scientific ltq xl getting started 176 pages brand. If you need additional guidance, refer to ltq xl online help, ltq xl getting connected, ion max api source hardware manual, or the ltq xl hardware manual. Schematic of the ltq orbitrap velos ms instrument with three new hardware implementations.
Browse through our guides and tools section to access a comprehensive portfolio of productrelated support resources. The ltq orbitrap velos hardware manualcontains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed. A triple quadrupole adds selected reaction monitoring. An orbitrap analyzer for fourier transformation b ased analysis. A, the stacked ring ion guide slens increases the ion flux from the electrospray ion source into the instrument by a factor 510. The orbitrap is also an ft instrument producing immense mass resolution whereas instruments like the saturn produced much lower resolution and mass accuracy. Etd module hardware manual for the ltq xl and ltq velos ms detectors compatible with ltq orbitrap xl and ltq orbitrap velos 9800097004 revision b april 2009. Ltq orbitrap series preinstallation requirements guide orbitrap elite getting started guide velos pro manual set you can access pdf files of the docume nts listed above an d of this guide from the data system computer. Ltq orbitrap velos hardware manual brown university. Who uses this guide this orbitrap velos pro hardware manual is intended for all personnel. Lipid analysis untargeted lipidomics, shotgun lipidomics. The orbitrap is an electrostatic or orbital trap and one of a kind.
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